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KMID : 0358419960390010146
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 1 p.146 ~ p.157
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The Effect of Bovine Serum Albumin on the Growth and Development of Preimplantation Mouse Embryo
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이미자
김향미/손영수/유한기/우복희
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Abstract
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This study was carried out to investigate the sensitivity of preimplantation mouse embryo to the influence of water quality and whether the influence of poor water quality is overcome by the addition of BSA.
F1 hybrid mice was superovulated with PMSG/hCG and timely mated. 1-cell stage and 2-cell stage mouse embryos were recruited. The embryos were cultivated in m-KRB and Ham's F-10 media; both media were produced by using highly purified water and
tap
water
and each media was divided into two groups: 0.4% BSA was added to one group and nothing was added to other group.
1-cell stage mouse embryo grew and developed invitro in simple media and complex media regardless of water quality. And its in vitro growth and development was significantly stimulated by the addition of BSA.
In simple media, in vitro growth and development of 2-cell mouse embryo was low in poor water quality but. The retarded growth and development was overcome by the addition of BSA.
In complex media, its in vitro growth and development was not changed by water quality but it was significantly stimulated by the addition of BSA.
Conclusively, in vitro growth and development of preimplantation mouse embryo is stimulated by the addition of BSA regardless of embryonic cell stage or media type. 2-cell stage mouse embryo appears to be sensitive to the influence of poor
quality,
especially in simple media, but it would be overcome by the addition of BSA.
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